Hunting genes, hunting endophenotypes

Hunting for differentially expressed genes.

Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many...

Hunting for Functionally Analogous Genes

Evidence indicates that members of many gene families in the genome of an organism tend to have homologues both within their own genome and in the genomes of other organisms. Amongst these homologues, typically only one or a few per genome perform an analogous function in their genome. Finding subsets of these genes which show evidence of performing a common function is an important rst step to...

Hunting genes from the Antarctic soil metagenome

Metagenomic library pp was obtained from Antarctic soil samples. Both functional and genotypic metagenomic screening were used for the isolation of novel cold-adapted enzymes with potential applications, and for the detection of genetic elements associated with gene mobilization, respectively. Fourteen lipase/esterase-, 4 amylase-, 3 protease-, and cellulase-producing clones were detected by ac...

Hunting Autoimmune Disease Genes in NOD:

Mutualistic hunting

The cooperative acquisition, defense and distribution of meat are common problems that must be solved by social hunters. These were also problems faced by any of our hominid ancestors that may have hunted large game in our evolutionary past. Much of the last 40 years of research in evolutionary ecology has tried to understand such cooperative behavior. The classic Prisoner's Dilemma game theory...